Open AccessStudy on the growth of Agrobacterium rhizogenes-induced Catharanthus roseus hairy roots in liquid media
Author(s) -
Nguyễn Như Nhứt,
Bùi Văn Lệ
Publication year - 2019
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v2i1.667
Subject(s) - catharanthus roseus , agrobacterium , sucrose , hairy root culture , botany , liquid culture , biology , biomass (ecology) , horticulture , liquid medium , chemistry , transformation (genetics) , food science , chromatography , agronomy , biochemistry , gene
Catharanthus roseus hairy roots induced by Agrobacterium rhizogenes get potential to apply to many fields. The suitable conditions for culturing depend on each hairy root line. In shaken liquid media in dark at 25 oC, VIN002-12 and VIN005-07 hairy root lines had the best growth in hemi-concentrated Gamborg’B5 media while VIN072-15 and VIN077-09 lines showed the best growth in in hemi-concentrated White media. The appropriate initial pH of medium for the lines was in range of 5.7–6.5. The hairy root lines used 2–5% sucrose as an optimal source of carbon for their growth. In selected conditions, the growth kinetics curves showed the end of exponential phase at the 28th day of culture with VIN005-07 and VIN072- 15 lines whereas at the 35th day with VIN002-12 and VIN077-09 lines. The initial results are quite possible to produce biomass for researches on four hairy root lines in the future.