Open AccessPreparation of chitosan microspheres by emulsion crosslinking method for biomedical application
Author(s) -
Anh Thi Tram Tu,
Hieu Van Le,
Huy Duc Ha
Publication year - 2017
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v1it4.491
Subject(s) - chitosan , glutaraldehyde , materials science , homogenization (climate) , emulsion , fourier transform infrared spectroscopy , molar ratio , polymer , microsphere , chemical engineering , dynamic light scattering , composite material , nanotechnology , chromatography , chemistry , nanoparticle , organic chemistry , biodiversity , ecology , engineering , biology , catalysis
The emulsion crosslinking method using glutaraldehyde as a crosslinking agent was exploited to prepare chitosan microspheres. Effects of preparation factors such as homogenization speed, oil/emulsifier ratio (O/E), oil/water ratio (O/W), crosslinker concentration on the structure of products were investigated. Structure and morphology of the obtained products were determined by Fourier transform infrared spectroscopy (FT-IR), Trasmission Optical Microscope systems (TOM), and Dynamic Laser Scattering analysis (DLS). The results indicated that the obtained products were spherical in shape. The average diameter of chitosan microspheres decreased with the increase of homogenization speed, O/W ratio, crosslinker concentration or decrease of O/E ratio. When homogenization speed is 6000 rpm, O/E =1.5, O/W=15, and molar ratio of chitosan to glutaraldehyde R=1, the average diameter of the chitosan microspheres was about 2.40 μm with a narrow size distribution. Chitosan microspheres are able to be used as a polymer matrix for targeted drug delivery systems due to their pH-responsive property.