Open AccessStudy on the in vitro growth of Lily Sorbonne bulblet
Author(s) -
Thắng Thanh Trần,
Hương Thanh Trần
Publication year - 2017
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v1it1.433
Subject(s) - zeatin , starch , shoot , sucrose , biology , botany , parenchyma , horticulture , growth rate , cytokinin , food science , biochemistry , gene , geometry , auxin , mathematics
In this study, the effects of light and dark condition, sucrose concentration, and plant growth regulators on the in vitro growth of bulblet from Lily Sorbonne shoot were studied. Morphological, physiological and biochemical changes in the bulblet growth were analyzed. Shoots in the light condition developed into plant while shoots in continuous darkness formed bulblet. Sucrose (90 g/L) increased the size of scale and starch accumulation of bulb. 6-Benzylaminopurine (BA 1,5 mg/L) stimulated the divison of parenchyma cell. The combination of 90 g/L sucrose and 1,5 mg/L BA in dark condition stimulated the division and size of the parenchyma cells, which increase the number and size of scales and size and weight of the bulblet. Respiratory rate, zeatin content and starch accumulation were increased in the bulblet growth. The correlation of light condition, sucrose concentration, plant hormone, respiration rate, starch accumulation, and bulblet growth were discussed. The combination of 90 g/L sucrose, 1.5 mg/L BA and 0.5 zeatin mg/L in dark strongly induced the growth of in vitro Lily bulblet, especially the number of scale.