Open AccessMolecular typing protocol of ss469415590 in IFNL4 gene in relation to the clearance of hepatitis C virus
Author(s) -
Huy Nguyen,
Linh T Tran,
Hung Huu Nguyen,
Chuong Hoang Nguyen
Publication year - 2017
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v1it1.431
Subject(s) - allele , typing , taqman , virology , biology , microbiology and biotechnology , genetics , primer (cosmetics) , gene , polymerase chain reaction , chemistry , organic chemistry
We built a molecular typing protocol of the ss469415590 SNP in the IFNL4 gene based on the real-time PCR technique with two Taqman probes which are specific for each allele of ss469415590. The protocol includes: (1) DNA extraction from whole blood; (2) DNA amplification in real-time PCR reactions with the primer pair of ss469415590_IFNL4_F - ss469415590_IFNL4_R and two speific Taqman probes ss469415590_IFNL4_FAM for the G allele and ss469415590_IFNL4_VIC for the TT allele. The typing protocol was evaluated on 95 clinical DNA samples. The allele frequencies were calculated as 93 % for the TT allele and 7 % for the G allele. The comparison of the typing protocol to the sequencing method revealed 100 % identical results.