Open AccessPreliminary study on the preservation of Catharanthus roseus (L.) G. Don hairy root lines induced by Agrobacterium rhizogenes isolated in Vietnam
Author(s) -
Nhut Nhu Nguyen,
Bùi Văn Lệ
Publication year - 2018
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v1i6.619
Subject(s) - catharanthus roseus , agrobacterium , hairy root culture , botany , biology , rhizobiaceae , horticulture , transformation (genetics) , gene , symbiosis , genetics , bacteria
Agrobacterium rhizogenes-transformed hairy roots from Catharanthus roseus have been widely used in research and in life. Consequently, methods for preservation are essential to maintain valuable hairy root lines. Our results showed that Gamborg’B5 was the most suitable medium for hairy roots from VIN002 and VIN005 while solid White media was more comfortable for the hairy lines from VIN022 and VIN077. All hairy root lines must be preserved in the dark at 25–27 oC. Under suitable conditions, the rate of lines growing normally reached 69.3, 67.0, 57.3 and 60.7 % for VIN002, VIN005, VIN072, and VIN077, respectively. There were 90.0, 93.3, 80.0 and 93.3 % of lines could preserve rolB, a gene which has important effect on the growth of hairy roots.