Open AccessRolesof plant growth regulators on the shoot development from the shoot apical meristem of Eustoma grandiflorum (RAF.) shinners
Author(s) -
Huyen Thach Quynh Ngo,
Hương Thanh Trần,
Viet Trang Bui
Publication year - 2018
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v1i6.616
Subject(s) - shoot , auxin , meristem , gibberellic acid , explant culture , kinetin , biology , botany , cytokinin , murashige and skoog medium , in vitro , germination , biochemistry , gene
In this paper, plant growth regulators including 6-benzylaminopurine (BA), kinetin, indole-3-acetic acid (IAA), gibberellic acid (GA3) and ethrel, at different concentrations were used individually or in combination to induce adventitious shoots from the explants, which contain shoot apical meristem and young leaves. Histological and physiological changes during shoot development were analysed. The highest shoot initiation was achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L BA and 1.0 mg/L GA3. Regenerated shoots were rooted on MS medium with 0.25 or 0.5 mg/L IAA. Shoot development from in vitro shoot explants initiated from the axil and cortex of stem. The shoot regeneration from shoot apical explants was effected by the meristem integrity or auxin from shoot apical meristem. Roles of plant growth regulators, especially polar auxin transport, and the ablation on the shoot initiation were discussed.