Open AccessEngineering yellow fluorescent protein probe for visualization of parallel DNA G-quadruplex
Author(s) -
Thanh Truong,
Trang P.T Phan,
Linh Le,
Dung Nguyen,
Hoàng Văn Nguyễn,
Dũng Đặng
Publication year - 2018
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v21i3.461
Subject(s) - g quadruplex , green fluorescent protein , fluorescence , chemistry , in vitro , dna , biophysics , yellow fluorescent protein , peptide , microbiology and biotechnology , computational biology , nanotechnology , biochemistry , biology , materials science , gene , physics , quantum mechanics
The formation of G-quadruplex plays a key role in many biological processes. Therefore, visualization of G-quadruplex is highly essential for design of G-quadruplex-targeted small molecules (drugs). Herein, we report on an engineered fluorescent protein probe which was able to distinguish G-quadruplex topologies.
Methods: The fluorescent protein probe was generated by genetically incorporating yellow fluorescent protein (YFP) to RNA helicase associated with AU-rich element (RHAU) peptide motif.
Results: This probe could selectively bind and visualize parallel G-quadruplex structure (T95-2T) at high affinity (Kd~130 nM). Visualization of the parallel G-quadruplex by RHAU-YFP could be easily observed in vitro by using normal Gel Doc or the naked eye.
Conclusion: The YFP probe could be encoded in cells to provide a powerful tool for detection of parallel G-quadruplexes both in vitro and in vivo.