Open AccessInhitive ability collagenase of N-(cinnamyl) chitooligosaccharide derivative
Author(s) -
Xuan Minh Le,
Khanh Nguyen,
Khoa D. Tran,
Quoc Tuan Tran,
DaiHung Ngo
Publication year - 2017
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v20ik3.1096
Subject(s) - collagenase , chemistry , cytotoxicity , hydrolysis , acetylation , chitosan , enzyme , matrix metalloproteinase , biochemistry , in vitro , gene
Chitooligosaccharides (COS) with molecular weight 4633 Da and 84.67% of deacetylation were synthesized by hydrolysis of chitosan by cellulase at room temperature (33 ± 1 ° C). This COS, then, were chemically modified by grafting cinnamaldehyde at amino groups on the COS. Derivatives of N- (cinnamyl) chitooligosaccharides (CCOS) synthesized with 50.64% of yield and 72.22% of extent of substitution had inhibitory activity enzyme collagenase (a group of matrix metalloproteinases, enzyme family related to metastatic ability of cancer). Compared with the positive control, 58.23% of the effective to inhibit collagenase of CCOS at 1000 μg/ml concentrate. In addition, the CCOS cytotoxicity of CCOS was also assessed by MTT method, the results showed that non-toxic derivatives of animal cells and thus can be tested and applied in living systems.