Open AccessOptimization of Chitosan hydrolysis by Cellulase Enzyme to produce Chitooligosaccharide
Author(s) -
Hoai Van Bui,
Quang An Dao,
DaiHung Ngo
Publication year - 2017
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v20ik3.1095
Subject(s) - cellulase , chitosan , hydrolysis , response surface methodology , central composite design , chemistry , enzymatic hydrolysis , solubility , chromatography , substrate (aquarium) , food science , biochemistry , organic chemistry , biology , ecology
In order to increase the water solubility of chitosan and potential application to products that is good for human health. Chitosan was caried out the optimization of hydrolysis by cellulase to produce chitooligosaccharide. Chitosan with degree of deacetyl more than 80% and cellulase used in this study. Surface Response Method (RSM) - Central Composite Design (CCD) option is used to optimize the hydrolysis. Results of the study showed optimal values for hydrolysis such as 49 oC temperature, 5.9 pH, 0.76% substrate concentration, and 8.97 U/g enzyme concentration, 180 minutes hydrolysis time. More than 90% of the oligosaccharides produced were in the range less than 10 kDa. The research results are the premise for production of COS powder to water-soluble.