Open AccessInvestigation of the antibiotic resistance prevalence and genes encoding exfoliative toxins of Staphylococcus aureus isolated from Pasteur institude HCMC
Author(s) -
Trang Nu Dai Phan,
Lan Le Ngoc Vu,
Ninh Nguyen Duc Uong,
Nghia Huu Cao
Publication year - 2016
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v19i2.755
Subject(s) - staphylococcus aureus , microbiology and biotechnology , antibiotics , staphylococcal scalded skin syndrome , antibiotic resistance , gene , biology , medicine , bacteria , genetics
Exfoliative toxins (ETs), the toxin caused the dangerous Staphylococcal scalded skin syndrome (SSSS), mainly affect on infants and young children [1]. By examining the ratio of antibiotic resistance and the detection of rate eta, etb genes encoding ETs toxins that cause disease in the group of Staphylococcus aureus (S. aureus), we would like to draw conclusions about the relationship between the disease gene with the antibiotic resistance of S. aureus thereby affording an appropriate choice of antibiotic to physicians, reduce the risk of spreading Methicillin resistant Staphylococcus aureus (MRSA) strains present in the community. In this study, we determine antibiotic susceptibility of S. aureus by Kirby-Bauer method on 293 S. aureus isolates from clinical specimens at the Department of Clinical Biology, Pasteur Institute HCMC. In total 293 S. aureus strains studied, we identified 49.7 % of MRSA strains the conduction PCR with primers specific detection of eta, etb gene that encoding toxins ETs applying on 118 strains S. aureus, discovered 4/118 (3.4 %) S. aureus trains carrying the gene eta, 1/118 (0.8 %) S. aureus trains carrying the gene etb. Because of the small presence of S. aureus strains carrying the genes encoding ETs the relationship between the gene for ET toxins and antibiotic resistance in S. aureus was not clearly defined.