Open AccessDevelopment of INCAT-GC-MS for analysis of metabolites in urine
Author(s) -
Peter Podolec,
Alexandra Hengerics Szabó,
Viktória Ferenczy,
Peter Kotora,
Jaroslav Blaško,
Róbert Kubinec,
Stanislav Stuchlı́k,
Martin Juriga,
Radomír Čabala,
Thanh Manh Ngo
Publication year - 2016
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v19i1.615
Subject(s) - urine , chromatography , sorbent , chemistry , gas chromatography–mass spectrometry , adsorption , metabolomics , mass spectrometry , biochemistry , organic chemistry
This paper aims to develop a non-solvent microextraction technique using INCAT (Inside Needle Capillary Adsorption Trap) for GC-MS analysis of volatile metabolites in urine. INCATs with different sorbents were tested, resulting in an INCAT with 3 sorbent layers Chromosorb W+ 20% SE-54, Carbopack X and Carboxen 1000 (1 : 1 : 1), which proved to be the most suitable for differentiating and identifying volatile metabolites in urine samples of patients from those of healthy people.