Development of INCAT-GC-MS for analysis of metabolites in urine | Zendy

Peter Podolec | Zendy; Alexandra Hengerics Szabó | Zendy; Viktória Ferenczy | Zendy; Peter Kotora | Zendy; Jaroslav Blaško | Zendy; Róbert Kubinec | Zendy; Stanislav Stuchlı́k | Zendy; Martin Juriga | Zendy; Radomír Čabala | Zendy; Thanh Manh Ngo | Zendy

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Development of INCAT-GC-MS for analysis of metabolites in urine

Author(s) -

Peter Podolec,

Alexandra Hengerics Szabó,

Viktória Ferenczy,

Peter Kotora,

Jaroslav Blaško,

Róbert Kubinec,

Stanislav Stuchlı́k,

Martin Juriga,

Radomír Čabala,

Thanh Manh Ngo

Publication year - 2016

Publication title -

science and technology development journal

Language(s) - English

Resource type - Journals

ISSN - 1859-0128

DOI - 10.32508/stdj.v19i1.615

Subject(s) - urine , chromatography , sorbent , chemistry , gas chromatography–mass spectrometry , adsorption , metabolomics , mass spectrometry , biochemistry , organic chemistry

This paper aims to develop a non-solvent microextraction technique using INCAT (Inside Needle Capillary Adsorption Trap) for GC-MS analysis of volatile metabolites in urine. INCATs with different sorbents were tested, resulting in an INCAT with 3 sorbent layers Chromosorb W+ 20% SE-54, Carbopack X and Carboxen 1000 (1 : 1 : 1), which proved to be the most suitable for differentiating and identifying volatile metabolites in urine samples of patients from those of healthy people.

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