Open AccessMolecular phylogenetic analysis to support the identification of samples DL0038A & DL0038B belonging to Cordyceps genus
Author(s) -
Luyen Tien Vu,
Hiep Minh Dinh,
Nguyen Binh Truong,
Thuan Duc Lao,
Hanh Van Trinh,
Thuy Ai Huyen Le
Publication year - 2016
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v19i1.544
Subject(s) - phylogenetic tree , cordyceps , biology , gene , ribosomal rna , sequence analysis , genetics , polymerase chain reaction , protein subunit , microbiology and biotechnology , botany
In our previous publication, we have tentatively concluded that our fungal specimen DL0038A and DL0038B are Cordyceps takaomontana. In order to further support the identification, we continued to analyse the nrSSU (nuclear ribosomal small subunit) and rpb1 (largest subunit of RNA polymerase II) genes, as well as combined analysis the nrLSU (largest subunit of RNA polymerase II) together with nrSSU and rpb2 genes on these specimens in order to support the morphological identification of those fungi. The results show that we successfully amplified all genes. Sequencing method was then adopted and proofread before molecular phylogenetic analysis was applied with reference sequences obtained from the publication of Sung et al. (2007). Once again, this analysis strongly supports the DL0038A and DL0038B specimen as Cordyceps takaomontana.