Open AccessIn vitro transfer of carbapenem resistant genes from Klebsiella pneumoniae to Escherichia coli J53
Author(s) -
Phuong Nhat Tran,
Thuoc Linh Tran,
Van Hung Pham
Publication year - 2016
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v19i1.542
Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , escherichia coli , biology , multiplex polymerase chain reaction , gene , antibiotic resistance , carbapenem , bacteria , antibiotics , virology , polymerase chain reaction , genetics
Nocosomial pathogen Klebsiella pneumoniae is a gram - negative bacterium that carries multiple antimicrobial resistant genes. Conjugative method was used for investigating of gene transfer from clinical carbapenem-resistant K. pneumoniae isolates to a recipient E. coli J53 in vitro. Multiplex PCR & Real-time PCR was used for detection of transferable genes among these strains. Transconjugants showed resistance to multiple antibiotics due to the presence of ESBLs & AmpC -lactamase as well as carbapenemase encoding genes. This is the great concern in Vietnam because resistant E. coli may become part of the normal gut flora and thereby a notable source of infections among sick and healthy persons in healthcare settings and in the community.