Open AccessStudy on synthesis of colloidal gold nanoparticles and preparation of antiE. coli O157 monoclonal antibody conjugated nanoparticles
Author(s) -
Thanh Vo Ke Ngo,
Giang Dang Nguyen,
Vinh Lam,
Duy C. Huynh
Publication year - 2013
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v16i3.1640
Subject(s) - chloroauric acid , colloidal gold , nanoparticle , conjugated system , ultraviolet visible spectroscopy , transmission electron microscopy , sodium citrate , nuclear chemistry , absorption spectroscopy , materials science , chemistry , nanotechnology , organic chemistry , polymer , medicine , physics , pathology , quantum mechanics
The present paper describes the preparation of colloidal gold nanoparticles by a chemical reduction method, using some chemical compounds such as sodium citrate (Na3Ct), chloroauric acid (HAuCl4.3H2O) and anti-E. coli O157 monoclonal antibodyconjugated gold nanoparticles. The nanoparticles were characterized by several techniques including Ultraviolet and Visible Absorption Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM). The UV-Vis confirmed that the concentration of Na3Ct has a major effect on the size of the synthesized gold nanoparticles, and TEM images showed that the size of gold particles is in the range of 10-20 nm. In addition, the binding of the antibody-gold conjugates to E. coli O157 was also investigated using the same characterization means