Open AccessIdentification of Basidiomycetes laccase genes in Nam Cat Tien forest soil by metagenomics
Author(s) -
Khanh Quoc Hoang,
Ngoc Bich Nguyen
Publication year - 2013
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v16i3.1639
Subject(s) - metagenomics , laccase , microorganism , biology , identification (biology) , gene , soil microbiology , cloning (programming) , botany , microbiology and biotechnology , computational biology , bacteria , genetics , enzyme , biochemistry , computer science , programming language
It was reported that there were 0.1 – 1% microorganism discovered by traditional cultivation, 99% others were not known cause of difficuties or impossible for growing in labratory’s conditions. Studying on microorganisms without culture was an aim of modern microbiology diversity. This research was used metagenomics method to access the diversity of laccase genes of mushrooms in Nam Cat Tien forest soil. The technique was simple, fast and cheap such as extracting and purifing DNA directly from soil, cloning by using retrograde primers and analyzing sequences of genes library. We have identified successfully laccase-like gene from samples collected in Nam Cat Tien forest.