Open AccessTransformation of callus sugarcane (Saccharum officinarum L.) using Agrobacterium tumefaciens – Mediated method and regeneration of transgenic shoot
Author(s) -
Ha Van Tran,
Phuong Hoang Phi Cung,
Bùi Văn Lệ
Publication year - 2013
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v16i1.1398
Subject(s) - acetosyringone , saccharum officinarum , callus , shoot , agrobacterium tumefaciens , explant culture , biology , inoculation , botany , transformation (genetics) , horticulture , genetically modified crops , murashige and skoog medium , transgene , gene , in vitro , biochemistry
Sugarcane (Saccharum officinarum L.) is a major industrial crop in the world. The creation of transgenic sugacane varieties contributed to overcome the disadvantages of traditional farming methods. Immature cutting cane stems after 10 days of putting on MS medium (Murashige and Skoog) supplemented 2,4-dichlorophenoxyacetic acid (2,4-D) 3 mg/L triggered the highest rate of explants inducing callus (91.11%). Calli were cultured on MS medium supplemented 6- Benzyladenin (BA) with concentrations of 0, 0.5, 1.0, 1.5, 2.0, 2.5 mg/L for inducing shoot. All six concentrations of BA could induce shoot, but the concentration of BA 1 mg/L was the most suitable. The 4-to-5-week old calli were introduced desired gene by using Agrobacterium tumefaciens, which was affected by acetosyringone concentration, inoculation and co-culture time. Consequently, transformation with acetosyringone 100 μM or 150 μM could be successful and regenerate transgenic shoots with assumed rate 25%.