Cloning and expression of recombinant human leptin in Escherichia coli | Zendy

Xuan Mai Huong Le | Zendy; To Dinh Le | Zendy; Thao Thi Phuong Dang | Zendy; Thuoc Linh Tran | Zendy

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Cloning and expression of recombinant human leptin in Escherichia coli

Author(s) -

Xuan Mai Huong Le,

To Dinh Le,

Thao Thi Phuong Dang,

Thuoc Linh Tran

Publication year - 2013

Publication title -

science and technology development journal

Language(s) - English

Resource type - Journals

ISSN - 1859-0128

DOI - 10.32508/stdj.v16i1.1391

Subject(s) - recombinant dna , leptin , escherichia coli , cloning (programming) , biology , inclusion bodies , expression vector , gene , microbiology and biotechnology , endocrinology , biochemistry , obesity , computer science , programming language

Leptin, a peptide hormone, is produced by mature adipocytes and functions primarily in the hypothalamus to reduce food intake and body weight. Recombinant h-leptin has been shown to be effective in obesity treatment. To overexpression of recombinant human leptin in Escherichia coli, the human leptin gene (hob gene) was cloned into the vector pET-28a. When analysis expression of human leptin in E. coli BL21(DE3) strain, it was found that recombinant vector pET-hob expressed h-leptinproteins in cytoplasm, and mainly as insoluble inclusion bodies. This result will be the premise for researching to produce recombinant human leptin protein.

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