Open AccessSELECTION, PRODUCTION AND CHARACTERIZATION OF BACILLUS LIPASE
Author(s) -
Khoa D. Tran,
Huy Quang Lê,
DaiHung Ngo
Publication year - 2011
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v14i3.2053
Subject(s) - lipase , bacillus subtilis , chemistry , substrate (aquarium) , enzyme , food science , biochemistry , chromatography , biology , bacteria , ecology , genetics
The objective of this study is to screen Bacillus strains for high lipase production; from 10 strains of Bacillus subtilis OII (BS7) has ability the best lipase production. The maximum lipase was carried out using liquid culture medium at pH 7,0, substrate concentrations 1,2% (w/w) after 3 days. The optimum pH and temperature on lipase activity were found to be pH 10,0 and 60 oC respectively. Ion Mn2+, Ca2+ are the ions remain activity; active lipase was inhibited by Cu2+ , Zn2 ; solution of SDS 0,2 % decreases 95% activity. Molecular weight of lipase production by BS7 is approximate 23,8kDa. The results are preconditions for the research on recombinant lipase by Bacillus subtilis OII.