Open AccessPRELIMINARY STUDY ON PRESERVATION OF RECOMBINANT hG-CSF BY LYOPHILIZATION
Author(s) -
Xuan Mai Huong Le,
Khanh Cat Vuong,
Hương Thanh Trần,
Giao Ngoc Quynh Nguyen,
Thao Thi Phuong Dang,
Thuoc Linh Tran
Publication year - 2011
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v14i3.1989
Subject(s) - recombinant dna , cytokine , freeze drying , neutropenia , chemistry , biological activity , microbiology and biotechnology , biology , chromatography , immunology , chemotherapy , biochemistry , medicine , in vitro , gene
hG-CSF is a cytokine that stimulates the proliferation, differentiation, function of mature neutrophils and is generally used for treatment of neutropenia in cancer patients under chemotherapy or other diseased patients. After production process, E. coli-derived recombinant hGCSF is usually available as non-glycosylated, liquid protein, so that it’s often unstable, easy to lose their biological activity. In this study, we report the results of using lyophilization to storage recombinant hG-CSF. We found out the freezing conditions and the suitable protein volumes for freezedrying process. Furthermore, we also studied the effects of lyophilization on this protein by testing the structure with Native-PAGE, RP-HPLC, testing biological activity on M-NFS-60 cell lines and in mouse (Mus musculus).