Open AccessSTUDYING ON CALLUS FORMATION FROM CHILLI PLANTLET CAPSICUM SP. AND CAPSAICINOID ACCUMULATION IN VITRO
Author(s) -
Phuc Thanh Vo,
Tien Thi Thuy Le
Publication year - 2011
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v14i3.1985
Subject(s) - callus , kinetin , cotyledon , explant culture , hypocotyl , plantlet , botany , biology , murashige and skoog medium , horticulture , chemistry , in vitro , biochemistry
Callus was initiated from hypocotyls and cotyledons explants of chilli Capsicum sp. in vitro on MS medium with 0,5 mg/l kinetin and 2,4-D /NAA (1,0; 1,5; 2,0; 2,5 and 3,0 mg/l). Callus from cotyledon explants was induced in the dark better than in the light, whereas callus from hypocotyl explants was initiated in the light better than in the dark. Callus was more friable and grew faster on medium with 2,4-D and kinetin. MS medium with 3,0 mg/l 2,4-D and 0,5 mg/l kinetin was optimal for the growth of callus from cotyledon explants. Besides, callus from hypocotyl explants grew best on MS medium with 1,5 mg/l 2,4-D and 0,5 mg/l kinetin. Capsaicinoid from callus which was determined by thin layer chromatography was recognized in all treatment experiments.