Open AccessSOMATIC EMBRYOGENESIS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) VAR. KM297
Author(s) -
Kien Van Vu,
Sanh Du Nguyen
Publication year - 2011
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v14i3.1984
Subject(s) - cotyledon , somatic embryogenesis , picloram , zeatin , embryo , biology , inoculation , botany , somatic cell , manihot esculenta , horticulture , embryogenesis , cytokinin , auxin , microbiology and biotechnology , biochemistry , gene
Somatic embryos of cassava var. KM297 received from pieces of in vitro immature leaf lobes or cotyledon of somatic embryos, were induced on the MS medium supplemented with 8mg/l picloram after 13 days inoculation in the dark condition. Different states of embryo were obtained after 10 days cultured on MS medium supplemented with 0.1 mg/l BA and 0.01mg/l NAA, in the light condition. Role of endogenous AIA and Zeatin of the globular state of embryos was studied.