PREPARATION, PURIFICATION AND PROPERTIES OF LIPASE FROM HEPATOPANCREAS OF TRA (PANGASIUS) CATFISH | Zendy

Thy Bao Vuong | Zendy; Lam Bich Tran | Zendy; Duan Luu | Zendy

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PREPARATION, PURIFICATION AND PROPERTIES OF LIPASE FROM HEPATOPANCREAS OF TRA (PANGASIUS) CATFISH

Author(s) -

Thy Bao Vuong,

Lam Bich Tran,

Duan Luu

Publication year - 2011

Publication title -

science and technology development journal

Language(s) - English

Resource type - Journals

ISSN - 1859-0128

DOI - 10.32508/stdj.v14i3.1959

Subject(s) - chromatography , lipase , catfish , chemistry , size exclusion chromatography , sephadex , hepatopancreas , fractionation , pangasius , enzyme , biochemistry , biology , fishery , fish <actinopterygii>

Lipase from the hepatopancreas of Tra (Pangasius) catfish was purified by ammonium sulfate fractionation, followed by ion-exhange chromatography on DEAE Cellulose and gel filtration Sephadex G-75. The preparation was homogeneous on polyacrylamide disc gel electrophoresis. The specific activity of the purified enzyme was 37.95 times higher than that of the crude extract. The enzyme showed a molecular weight of 57000 Da. The pH and temperature optima of purified lipase were 8 and 500C respectively. Enzyme activity was enhanced by Ca2+ but inhibited by heavy metals Zn2+, Cd2+, Mg2+.

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