Open AccessCOLLECT EXTRACELLULAR MATRIX FROM IN VITRO FIBROBLAST
Author(s) -
Giang Thi Thanh Nguyen,
Quan Minh To,
Ngoc Kim Phan,
Ha Le Bao Tran
Publication year - 2009
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v12i9.2280
Subject(s) - extracellular matrix , fibroblast , ascorbic acid , vimentin , masson's trichrome stain , in vitro , staining , microbiology and biotechnology , matrix (chemical analysis) , chemistry , tissue engineering , immunohistochemistry , pathology , biology , biochemistry , immunology , biomedical engineering , medicine , chromatography , food science
Extracellular matrices (ECM) have been reported to enhance cell attachment and proliferation as well as to create stem cell niches invitro. We harvested ECM from human fibroblasts for a number of researches, including tissue engineering. Fibroblasts were isolated from human foreskins, cultured in DMEMF12 containing 10% FBS and identified by Trichrome staining and immunohistochemistry for Vimentin. Then, fibroblasts were stimulated to synthetize ECM in medium supplemented 0.05% ascorbic acid. Cell constituents were removed by using Triton X-100, NH4OH and DNAse. ECM proteins were evaluated by PAS staining. Results showed that collagen is present in ECM.