Open AccessEFFECT OF PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS OF EGGPLANT (SOLANUM MELONGENA L.)
Author(s) -
Ngọc Nam Trịnh,
Sanh Du Nguyen
Publication year - 2009
Publication title -
khoa học công nghệ
Language(s) - English
Resource type - Journals
ISSN - 1859-0128
DOI - 10.32508/stdj.v12i17.2369
Subject(s) - somatic embryogenesis , kinetin , biology , callus , embryo , botany , 1 naphthaleneacetic acid , murashige and skoog medium , germination , horticulture , tissue culture , somatic cell , ethephon , auxin , embryogenesis , microbiology and biotechnology , in vitro , biochemistry , ethylene , gene , catalysis
On the MS medium containing only 2,4-D, callus induction was inhibited. Moreover, these calli were friable and turned brown after two weeks of culture. the three-week old calli were then transferred to the MS medium containing NAA 0.5 mg/l and kinetin 1 mg/l. The somatic embryos with globular shape appeared after 10 days of culture, while the heart shape, torpedo_shape and cotyledonary_shape embryos appeared successively after 15 days of culture. The abnormal embryos occupied at a rate of 34.3% and rarely germinated to plantlets. On the MS medium without plant growth regulators or only with NAA, somatic embryos could not be induced. On MS medium supplemented with ethephon 3 mg/l somatic embryogenesis from calli was inhibited. Plantlets derived from the eggplant somatic embryos had a survival rate up to 95% when transferred to the pots.