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Sperm cytology: Kernechtrot–picroindigocarmine stain and dog semen
Author(s) -
Adam W. Stern
Publication year - 2019
Publication title -
journal of veterinary forensic sciences
Language(s) - English
Resource type - Journals
ISSN - 2690-3660
DOI - 10.32473/jvfs.v1i1.128311
Subject(s) - sperm , stain , semen , staining , biology , andrology , anatomy , medicine , genetics
Animal sexual abuse cases present as an unusual scenario for forensic investigators. In this study, the kernechtrot–picroindigocarmine (KPIC, aka Christmas tree stain) staining properties of semen from 8 domestic dogs was compared to two samples of human sperm. In the head of the dog sperm, the acrosomal region stained pale red and the postacrosomal region stained dark red. There was a colorless band with a mean length of 0.59 μm within the sperm head between the acrosomal and postacrosomal regions. The colorless band was not identified within the human control samples nor reported in the literature. This data supports the use of KPIC stain for the examination of dog semen and further characterizes a unique staining pattern found in dog sperm that is not present in human sperm.

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