Open AccessTWO NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS IN THE VOLTAGE-GATED SODIUM CHANNEL GENE IDENTIFIED IN AEDES AEGYPTI MOSQUITOES FROM FLORIDA
Author(s) -
Kyle J. Kosinski,
Lee Y,
Ana L. Romero-Weaver,
Tse-yu Chen,
Travis C. Collier,
Xiaodi Wang,
Derrick Mathias,
Eva A. Buckner
Publication year - 2022
Publication title -
journal of the florida mosquito control association
Language(s) - English
Resource type - Journals
eISSN - 2638-6054
pISSN - 1055-355X
DOI - 10.32473/jfmca.v69i1.130622
Subject(s) - aedes aegypti , knockdown resistance , biology , single nucleotide polymorphism , genetics , pyrethroid , genotyping , nonsynonymous substitution , snp genotyping , gene , chikungunya , genotype , genome , virus , pesticide , larva , botany , cyfluthrin , agronomy
Aedes aegypti, the primary vector of dengue, Zika, chikungunya, and yellow fever viruses, is known to be resistant to pyrethroid-based insecticides in Florida. To improve our knowledge on the mechanism(s) responsible for this resistance, we sequenced 106 Ae. aegypti individuals collected from throughout Florida and examined mutations in a known insecticide resistance gene, voltage-gated sodium channel (VGSC; AAEL023266), also commonly known as the knockdown resistance (kdr) gene. Through whole genome sequencing, we identified 2 novel nonsynonymous single nucleotide polymorphisms (SNPs), F174I and E478K, and 5 known SNPs, V410L, S723T, V1016I, D1763Y, and Q1853R, of which 4 were reported in Floridian Ae. aegypti for the first time. These SNPs provide a basis for further studies examining their contribution to pyrethroid resistant phenotypes, such as increased time of survival after insecticide exposure. This sequence data can be used to develop a multiplex genotyping assay to investigate the SNP frequencies in a larger number of samples and to examine their phenotypic contribution to pyrethroid resistance in Ae. aegypti.