Open AccessFeatures of Borna disease virus reproduction in brain cells and laboratory diagnosis of infection
Author(s) -
Константин Юров,
K. P. Yurov,
Светлана Алексеенкова,
Светлана Алексеенкова
Publication year - 2019
Publication title -
rossijskij veterinarnyj žurnal
Language(s) - English
Resource type - Journals
ISSN - 2500-4379
DOI - 10.32416/article_5cd16d07159988.78868190
Subject(s) - endosome , axon , neurotropic virus , cytoplasm , virus , biology , axoplasmic transport , phosphoprotein , virology , microbiology and biotechnology , rna , pathology , medicine , genetics , phosphorylation , gene , intracellular
The Borna Disease Virus (BDV) is a non-segmented RNA-containing virus belonging to the Bornaviridae family. The pathogen causes progressive meningoencephalitis in animals of various types. Despite numerous studies, some stages of reproduction of Bornavirus remain poorly understood, in particular, receptor-mediated penetration, retrograde transport into the nucleus, assembly and release of the virion, etc. The present report presents the results of demonstrating studies that were aimed at following the immunoenzyme method, the distribution of the main protein BDV ― phosphoprotein P (p24) in the brain cells of naturally susceptible animals, compare nnye data with the results of a number of authors, made mainly in experimental models. Microscopic examination of histological sections of the brain tissue of horses and sheep treated with specific serum against p24 BDV in a light or luminescent microscope observed a specific color in the form of: small granules; diffuse fluorescence of the cytoplasm; larger granules, apparently due to aggregation of endosomes, for axon transportation; formations in the form of beads, demonstrating the transport of viral material along the axon. In vitro results were obtained indicating that it is possible to transport RNPs via a short path through cytoplasmic bridges. The presented results will allow a better understanding of the neuropathogenesis of Born's disease and improve the diagnosis of the disease.