Open AccessDetection of prion protein, the infection agent of bovine spongiform encephalopathy, by using aptamers
Author(s) -
Алексей Антипин,
Aleksey Antipin,
Григорий Надточей,
G. A. Nadtochey
Publication year - 2019
Publication title -
rossijskij veterinarnyj žurnal
Language(s) - English
Resource type - Journals
ISSN - 2500-4379
DOI - 10.32416/2500-4379-2019-2019-6-5-8
Subject(s) - aptamer , bovine spongiform encephalopathy , prion protein , dna , virology , biology , transmissible spongiform encephalopathy , microbiology and biotechnology , chemistry , pathology , biochemistry , scrapie , medicine , disease
In the paper results of the specific interaction of selected DNA aptamers with prion protein from CNS tissues from DSE infected cows are described. The test detects amyloid epitops.
The purpose of investigation. To study the possibility of detection of prion protein (BSE infectious agent in tissues CNS infected cows), using DNA aptamers. The DNA aptamers had been selected to yeast prions.
Materials and methods. CNS tissues samples from a DSE experimentally infected cow were used. The infection was confirmed by histological analyses. The prion protein of BSE was isolated by ultracentrifugation in sucrose gradient and analysed, using immunoblotting and electron microscopy.
Results. DSE infection in an experimentally infected cow was confirmed. The brain tissue samples were prepared. Specific effective binding of selected DNA aptamers with prions from DSE cow brain tissues was revealed.
Conclusion. The possibility of detection of prion proteins in brain tissues from a DSE cow, using DNA aptamers, specifically binded with amyloid epitops was shown.