Open AccessBiologic Characteristics of Bone Substituting Tissue Engineering Construction Based on Calcium Phosphate Ceramics, Autologous Mesenchymal Stromal Cells and Fibrin Hydrogel
Author(s) -
Vasily Mamonov,
A. G. Chemis,
В. С. Комлев,
A. L. Berkovskiy,
Е.М. Голубев,
N. V. Proskurina,
Natalia Sats,
Nina Drize
Publication year - 2015
Publication title -
vestnik travmatologii i ortopedii imeni n.n. priorova
Language(s) - English
Resource type - Journals
eISSN - 2658-6738
pISSN - 0869-8678
DOI - 10.32414/0869-8678-2015-4-52-59
Subject(s) - mesenchymal stem cell , fibrin , biomedical engineering , calcium , chemistry , tissue engineering , tec , stromal cell , regeneration (biology) , biomaterial , in vivo , microbiology and biotechnology , materials science , pathology , biology , immunology , medicine , ionosphere , physics , organic chemistry , astronomy
Biological characteristics of bone substituting tissue engineering construction (TEC) that contained porous calcium phosphate ceramic granulate (CPC) of phase structure ((tricalcium phosphate (TCP)), fibrin hydrogel and autologous multipotent mesenchymal stromal cells (auto-MMSC) induced and non-induced to osteogenic differentiation were studied in vivo. The following characteristics of TEC were determined: ability to transfer within its structure the viable auto-MMSC with preservation of their regeneration potential; ability to osteogenesis only under conditions of orthotopic implantation; ability of induced to osteogenic differentiation auto-MMSC to participate in the reparative processes for not more than within 6 weeks after implantation; negative affect of fibrin hydrogel on the osteoinductive properties of CPC within TCP structure. It was shown that to provide osteogenesis in the implanted TEC not only the viable auto-MMSC but simultaneous presence of osteoinductive and osteoconductive factors was required. No bone formation in a critical bone defect and in ectopic implantation takes place without observance of these conditions.