Open AccessIn Vitro Evaluation of the Composite Alginate - Calcium Phosphate Materials for Prototyping Technologies in Bone Defects Substitution
Author(s) -
Н. С. Сергеева,
В. С. Комлев,
И. К. Свиридова,
В. А. Кирсанова,
С. А. Ахмедова,
Е. А. Кувшинова,
А. И. Федотов,
А. Ю. Тетерина,
С. М. Баринов
Publication year - 2015
Publication title -
vestnik travmatologii i ortopedii imeni n.n. priorova
Language(s) - English
Resource type - Journals
eISSN - 2658-6738
pISSN - 0869-8678
DOI - 10.32414/0869-8678-2015-1-28-34
Subject(s) - gelatin , sodium alginate , composite number , mtt assay , calcium alginate , adhesive , cytotoxicity , matrix (chemical analysis) , chemistry , in vitro , materials science , calcium , chemical engineering , biomedical engineering , sodium , composite material , biochemistry , organic chemistry , layer (electronics) , medicine , engineering
Porous granular composite biomaterials based on sodium alginate and calcium phosphate component: 1% sodium alginate; sodium alginate with gelatin (80/20); calcium phosphate ceramics (CHA, TCP and OCP) and composite materials alginate/CHA, alginate/TCP, alginate/OCP, alginate/gelatin (80/20)/TCP and alginate/TCP/CHA/OCP were investigated for cytotoxicity and matrix (for cells) properties in dynamics of cultivation (up to 21 days). Two adhesive cell lines - immortalized human fibroblasts (HF, strain 1608 hTERT) and human osteosarcoma (MG-63) were used for in vitro MTT assay. OCP material showed its cytotoxicity while all its composites possessed weak matrix properties. For further research on the development of prototyping technologies 3 samples of composite materials were selected: alginate/CHA, alginate/TCP and alginate/gelatin (80/20)/TCP that are characterized by cytocompatibity and the presence of either expressed or moderate matrix properties of the surface for cells.