Open AccessNew species within Candida parapsilosis and Candida glabrata
Author(s) -
Dariusz Domański,
Magdalena Sikora,
Robert Kuthan,
Ewa AugustynowiczKopeć,
Ewa Swoboda-Kopeć
Publication year - 2019
Publication title -
medycyna doświadczalna i mikrobiologia
Language(s) - English
Resource type - Journals
eISSN - 2545-2517
pISSN - 0025-8601
DOI - 10.32394/mdm.71.06
Subject(s) - candida glabrata , biology , candida parapsilosis , internal transcribed spacer , species complex , microbiology and biotechnology , sensu stricto , subgenus , restriction fragment length polymorphism , taxonomy (biology) , gene , candida albicans , genetics , ribosomal rna , polymerase chain reaction , zoology , phylogenetic tree
Candida parapsilosis and Candida glabrata are another yeasts that form complexes of crypospecies. Although these species have been described more than a decade ago, knowledge about them is still limited. The reason for this is the large phenotypic similarity that unables them from being differentiated by classical diagnostic methods. The aim of the study was to identify species of clinical strains within C. glabrata and C. parapsilosis complexes.Material and methods: Standard PCR-RFLP of the secondary alcohol dehydrogenase gene (SADH) with BanI restriction enzyme served to determine species affiliation within the C. parapsilosis complex. The internal transcribed spacer was used to confirm the identification of C. glabrata sensu stricto. The D1/D2 domain of the 26S rDNA gene was sequenced in order to identify C. nivariensis and C. bracarensis strains.Results: As a result of the molecular analysis, 24 Candida nivariensis isolates and 4 C. metapsilosis strains and 9 C. orthopsilosis strains were detected.Conclusions: Prevalence of new cryptic species was relatively low.