Open AccessINFLUENCE OF POLYMYXIN B ON THE FORMATION OF BIOFILMS BY BACTERIUM Methylophilus quaylei ON POLYPROPYLENE AND TEFLON
Author(s) -
А. М. Мохамед,
Д. Н. Амзаева,
А. Б. Пшеничникова,
В. И. Швец
Publication year - 2018
Publication title -
tonkie himičeskie tehnologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7575
pISSN - 2410-6593
DOI - 10.32362/2410-6593-2018-13-2-31-39
Subject(s) - biofilm , microbiology and biotechnology , polymyxin b , streptomycin , crystal violet , strain (injury) , chemistry , bacteria , polymyxin , polypropylene , antibiotics , biology , organic chemistry , anatomy , genetics
Here we examined the influence of polymyxin B, a lipopeptide antibiotic, on planktonic growth and biofilm of streptomycin-sensitive and streptomycin-resistant isogenic strains of bacteria Methylophilus quaylei on polypropylene and Teflon. Biofilm formation was quantified using crystal violet staining, determined by colony-forming unit and examined by light microscopy. It was found that of bacterial surface hydrophobicity affects the biofilm formation: biofilm formation of Methylophilus quaylei MT strain was better on such hydrophobic materials as Teflon and polypropylene. The minimum inhibitory concentration of polymyxin B for the used strains in planktonic and biofilm form was 1 μg/ml. The sublethal Polymyxin B in the concentration of 0.01 μg/ml stimulates biofilm formation and exhibits antibiofilm properties at the concentration of 0.10 μg/ml. Synergistic effect of polymyxin B and streptomycin on streptomycin-resistant strain M. quaylei SM was found.