Open AccessAPPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL
Author(s) -
Irina Zvereva,
N. B. Savelieva,
P. V. Postnikov,
Yu. A. Efimova,
М. А. Дикунец
Publication year - 2017
Publication title -
tonkie himičeskie tehnologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7575
pISSN - 2410-6593
DOI - 10.32362/2410-6593-2017-12-1-64-75
Subject(s) - human chorionic gonadotropin , tandem mass spectrometry , high performance liquid chromatography , chromatography , chemistry , mass spectrometry , gene isoform , equine chorionic gonadotropin , gonadotropin , resolution (logic) , hormone , biochemistry , gene , computer science , ovulation , artificial intelligence
The article presents the results of the first stage of development of a new quantitative method for human chorionic gonadotropin (hCG) determination by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) to uncover doping abuse by athletes. The identified tryptic peptides correspond to the most abundant hCG isoforms: the α- and β-subunits, the nicked and β-core fragment of the hormone. Identification and sequencing of specific fragments were performed with the use of nanoLC-MS/MS. A high resolution / high accuracy hybrid mass-spectrometer was applied. Optimization of mass-spectrometric determination of selected specific peptides was accomplished by UPLC-MS/MS. Quantitative evaluation of hCG using specific fragments determination by UPLC-MS/MS allows to detect corresponding hCG isoforms. This significantly increases the method specificity and decreases the probability of false-positive results.