Open AccessSingle Cell Sequencing, Its Application and Future Challenges
Author(s) -
Misbah Saleem,
Sadaf Alam,
Iqra Kainat,
Ramsha Iftikhar,
Aqsa Ijaz
Publication year - 2021
Publication title -
current trends in omics
Language(s) - English
Resource type - Journals
eISSN - 2790-8291
pISSN - 2790-8283
DOI - 10.32350/cto.11.05
Subject(s) - epigenome , single cell sequencing , biology , computational biology , dna sequencing , transcriptome , genome , epigenetics , gene , genetics , phenotype , exome sequencing , dna methylation , gene expression
Single-cell sequencing investigates the differences in proteomic and genetic information about individual cells by using next-generation sequencing technologies. Sequencing of the whole genome, epigenome, and transcriptome involves the heterogeneous process of diagnosis, progression, and treatment of disease. Previous studies show that only a few selected proteins and RNAs can be measured but recent molecular studies explore that advances in next-generation sequencing and whole genome amplification enabled us to examine the differences among a variety of transcriptomic cells, gene expression, and phenotypic expressions. In our study, we try to summarize different technologies and their applications at single-cell level in diverse fields such as embryology, oncology, immunology, neurology, microbiology, tissue and organ development, antibody screening, and stem cell research.