Open AccessTHROMBOSE-PREVENTIVE INFLUENCE OF QUERCETIN IN RATS ON THE HYPER-COAGULATION DIET
Author(s) -
V.S. Potaskalova,
M.V. Кhaitovych,
Olga Plenova,
Maria Valigura
Publication year - 2019
Publication title -
medična nauka ukraïni
Language(s) - English
Resource type - Journals
eISSN - 2664-4738
pISSN - 2664-472X
DOI - 10.32345/2664-4738.3-4.2019.03
Subject(s) - partial thromboplastin time , fibrinogen , prothrombin time , thrombin time , coagulation , fibrin , medicine , endocrinology , thromboplastin , platelet poor plasma , chemistry , platelet , thrombin , immunology
Relevance. Hyper-coagulation processes increase the risk of coronary heart disease.
Objective of the study was to study the effect of quercetin on the hemostatic system when using hyper-coagulation diet in rats.
Materials and methods. The study included 17 male rats weighing 240-270 g. The rats of the control group (n = 6) were fed with conventional compound feed; Group I rats (n = 5) - mixed feed; and group II rats (n = 6) mixed with meat and quercetin (1.33 g/l). After 21 days, sterile blood was collected. The Amellung Coagulometer KS 4A studied the external and internal blood coagulation pathways, the activity of their own anticoagulant and fibrinolytic blood systems. The data obtained were statistically processed using Excel 2010 and Statistica v.10 (StatSoft, USA).
Results. In rats of group 1, the external pathway of blood coagulation underwent the most significant changes, as indicated by an almost four-fold acceleration of prothrombin time and a decrease in the international normalized ratio. From the side of the internal pathway, the greatest changes were recorded in the last phase of clot formation: thrombin time decreased by 39.8% with an almost constant rate of activated partial thromboplastin time (APTT), which confirmed the activation of the fibrin formation phase. In addition, the content of soluble fibrin-monomer complexes (RFMC) increased 1.7 times and the fibrinogen decreased. At the same time, depletion of the anticoagulant potential of the blood was observed (a 42.2% decrease in the activity of the protein C system with a tendency to a decrease in antithrombin III (AT III). The fibrinolytic system was significantly suppressed, as indicated by more than three times inhibition of XII-dependent fibrinolysis. In animals Group 2 compared with the indicator in group 1, APTT was 2 times longer; the concentration of RFMC was also doubled, fibrinolysis was activated by 25%.
Conclusion: The use of quercetin bioflavonoids in animals with simulated coronary heart disease slows down blood coagulation and potentiates the anticoagulant system, including due to a 40% increase in AT III activity, can be considered as a thrombosis-preventive effect