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Application of real-time polymerase chain reaction in the clinical genetic practice
Author(s) -
Balázs Nagy
Publication year - 2015
Publication title -
journal of pediatric genetics
Language(s) - English
Resource type - Journals
eISSN - 2146-4596
pISSN - 2146-460X
DOI - 10.3233/pge-13042
Subject(s) - polymerase chain reaction , nucleic acid , genetics , biology , polymerase , computational biology , genome , digital polymerase chain reaction , gene , molecular diagnostics , nucleotide , microbiology and biotechnology
The development of polymerase chain reaction revolutionized the molecular genetics and diagnostics. Technical improvements helped to make more specific and sensitive target determinations. Introduction of real-time polymerase chain reaction makes possible several applications in clinical genetics like detection of gene mutations, single nucleotide polymorphisms, deletions, measurement of gene expressions, micro ribonucleic acids, free nucleic acids and microbial genomes. Here I discuss a few examples for specific applications in prenatal clinical genetic practice. These are the detection of microbial genomes, deletions, trisomies, mutations, single nucleotide polymorphisms and free nucleic acids.

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