Open AccessA Sensitive and Cost-Effective Chemiluminescence ELISA for Measurement of Amyloid-β 1-42 Peptide in Human Plasma
Author(s) -
Pankaj Mehta,
Bruce A. Patrick,
David L. Miller,
Patricia K. Coyle,
Thomas Wısnıewskı
Publication year - 2020
Publication title -
journal of alzheimer's disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.677
H-Index - 139
eISSN - 1875-8908
pISSN - 1387-2877
DOI - 10.3233/jad-200861
Subject(s) - immunoassay , detection limit , chemiluminescence , chemistry , monoclonal antibody , chemiluminescent immunoassay , antibody , peptide , substrate (aquarium) , human plasma , chromatography , reagent , microbiology and biotechnology , medicine , immunology , biochemistry , biology , ecology
Amyloid-β42 (Aβ42) is associated with plaque formation in the brain of patients with Alzheimer's disease (AD). Studies have suggested the potential utility of plasma Aβ42 levels in the diagnosis, and in longitudinal study of AD pathology. Conventional ELISAs are used to measure Aβ42 levels in plasma but are not sensitive enough to quantitate low levels. Although ultrasensitive assays like single molecule array or immunoprecipitation-mass spectrometry have been developed to quantitate plasma Aβ42 levels, the high cost of instruments and reagents limit their use.