Open AccessPOLYMORPHISMS IN THE GENES OF REPARATIONS AMONG EMPLOYEES OF THE ATOMIC INDUSTRY OF KAZAKHSTAN
Author(s) -
D. M. Botbаeyev,
А. M. Belkozhаev,
A. K. Khanseitova,
A. Zh. Borbayeva,
N. А. Аitkhozhinа
Publication year - 2020
Publication title -
izvestiâ nacionalʹnoj akademii nauk respubliki kazahstan. seriâ biologičeskaâ i medicinskaâ
Language(s) - English
Resource type - Journals
eISSN - 2518-1629
pISSN - 2224-5308
DOI - 10.32014/2020.2519-1629.1
Subject(s) - gene , genetics , genotype , single nucleotide polymorphism , allele , biology , intron , restriction fragment length polymorphism , polymorphism (computer science) , polymerase chain reaction , allele frequency , microbiology and biotechnology
Single nucleotide polymorphisms (SNPs) are the most convenient marker and the widespread subject of polymorphism testing. To identify the presence or absence of the effects of chronic low-dose radiation on nuclear industry personnel, the occurrence of single-nucleotide substitutions at the polymorphic sites of the genes of the repair system 3 and 6 of the introns of the APC gene P53.11 gene, in positions -2549 of the VEGF gene, XPD gene rs313181 ( Lys751Gln) and rs25487 of the XRCC gene (Аrg399Gln) were compared. Analysis of allele frequencies and distribution of genotypes in the variable regions of the tested genes was performed by the method of polymerase chain reaction (PCR), followed by determination of restriction fragment length polymorphism (RFLP). When com-paring the frequencies of alleles and the distribution of genotypes between the second group of miners (11–20 years’ experience) and control, differences in the distribution of genotypes in the rs25487 XRCC plot (χ2 = 7.11, p = 0.028) were revealed. These differences satisfy the criterion p <0.05 and, accordingly, are statistically significant.Key words: polymorphism, genes, a nuclear industry.