Open AccessRAW MATERIALS IDENTIFICATION AND MANUFACTURED PRODUCTS AUTHENTICATION TECHNOLOGIES
Author(s) -
Р. Р. Вафин,
I. Yu. Mikhailova,
V.K. Semipyatny,
Kh.Kh. Gilmanov,
А.В. Бигаева,
E.G. Lazareva
Publication year - 2020
Publication title -
izvestiâ nacionalʹnoj akademii nauk respubliki kazahstan. seriâ himii i tehnologii
Language(s) - English
Resource type - Journals
eISSN - 2518-1491
pISSN - 2224-5286
DOI - 10.32014/2020.2518-1491.106
Subject(s) - wine , raw material , authentication (law) , dna extraction , identification (biology) , microbiology and biotechnology , polymerase chain reaction , food science , pulp and paper industry , computational biology , computer science , biology , gene , botany , engineering , genetics , computer security , ecology
DNA technologies for the raw grapes identification and the authentication of wine products made from them are popular scientific and practical direction with the prospect of introducing developments into the quality management system. The molecular genetic approaches development to the technical grape varieties’ genetic identification and wines DNA authentication produced from them was the aim of this work. Sample preparation of experimental wines was carried out by exposure in a mixture with a precipitant and a co-precipitant, followed by wine debris precipitation. The nucleic acids extraction from wine debris was carried out with the same commercial kit as from the pulp of a mature grape fruit, but with an additional lysis solution modification. The obtained results indicated the selected conditions effectiveness for sample preparation, DNA isolation and PCR for amplification of the analyzed Vitis vinifera L. UFGT-gene loci. The described strategy for the raw materials geno-identification and the manufactured products authentication is based on the polymorphic positions of diagnostically significant UFGT-gene-associated groups analysis, interpreted by sequencing the corresponding PCR product.