Open AccessThe first detection of Botryosphaeria sinensis on white lupine
Author(s) -
Е. Л. Гасич,
А. С. Орина
Publication year - 2021
Publication title -
vestnik zaŝity rastenij
Language(s) - English
Resource type - Journals
eISSN - 2308-6459
pISSN - 1727-1320
DOI - 10.31993/2308-6459-2021-104-4-15142
Subject(s) - biology , internal transcribed spacer , mycelium , botany , strain (injury) , white (mutation) , fungus , phylogenetic tree , gene , genetics , anatomy
Fungal strain MF KP-12.1, which is similar in morphological and cultural characteristics to Botryosphaeria fungi, was isolated from the seeds of white lupine variety Dega grown in Oryol region in 2018. Phylogenetic analysis of the large rRNA subunit (LSU), elongation factor-1α (TEF) and internal transcribed spacer (ITS) fragments was used to accurately identify the isolated strain as B. sinensis. When 2-week-old white lupine plants were treated under laboratory conditions with mycelial suspension of B. sinensis MF KP-12.1, plant damage reached 67 % on day 3, and 100 % plant death was observed on day 10. Pathogenicity of B. sinensis MF KP-12.1 was confirmed by re-isolation of the strain from damaged plant tissue. This is the first detection of B. sinensis on white lupine, which has not been previously reported as a host for this fungus. It is also the first detection of B. sinensis in Russia.