Open AccessPreservation of Quercus Robur and Quercus Petraea Genetic Resources through In Vitro Culture
Author(s) -
Lucia Ioniță,
AUTHOR_ID,
Ionel Mirancea,
Ecateriicoleta Apostol,
M. Budeanu,
Emanuel BESLIU,
AUTHOR_ID,
AUTHOR_ID,
AUTHOR_ID,
AUTHOR_ID
Publication year - 2021
Publication title -
bulletin of the "transilvania" university of braşov. series ii, forestry, wood industry, agricultural food engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.207
H-Index - 6
eISSN - 2065-2143
pISSN - 2065-2135
DOI - 10.31926/but.fwiafe.2021.14.63.2.2
Subject(s) - explant culture , quercus robur , sterilization (economics) , biology , botany , in vitro , ascorbic acid , horticulture , germination , clone (java method) , quercus petraea , tissue culture , biochemistry , dna , economics , monetary economics , foreign exchange market , foreign exchange
In this paper, the effect of various factors (clone, initial explant, sterilization method, and culture medium) on the in vitro multiplication of pedunculate and sessile oak species was tested. No influence of clone was found and a 44-83% survival rate was found for different types of explants, the best result being that of using mature acorns germinated in vitro. The most efficient sterilization method was: mercuric chloride 0.2 mg/l, ascorbic acid 1 mg/l, for 30 min., with or without antibiotic. The best results were obtained with GD medium complemented with 0.5 mg/l BAP and 0.1 mg/l IBA, which allowed obtaining of a high rooting percentage. The regenerated plants were transferred in vivo, in a climate controlled greenhouse.