Open AccessNew method of complex photo-fluorescent microbiotesting
Author(s) -
V. S. Sibirtsev,
V. S. Sibirtsev,
A. V. Garabadgiu,
А. В. Гарабаджиу,
В. И. Швец,
В. И. Швец
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524896641-645
Subject(s) - fluorescence , biological system , structuring , absorption (acoustics) , fluorescent light , component (thermodynamics) , chemistry , light scattering , scattering , materials science , chromatography , optics , biology , physics , finance , economics , thermodynamics
The biotesting procedure is described, which provides for recording changes in the intensities of elastic light scattering, light absorption and intrinsic photofluorescence of the protein component, as well as determination of concentration and structuring coefficients of the genomic component of samples with viable unicellular test organisms, incubated in a liquid nutrient medium in the presence and absence of various external chemical factors. The results of the analysis using this technique of the antibiotic activity of cations of various metals are presented. It is shown that using this technique can be much more rapid, objectively and comprehensively than using standard visual microbiotesting methods, to assess the effect on reproduction rate, metabolic activity and genome structure of test organisms of samples of various products, wastes, etc.