Open AccessResistance of THP-1 leukemia cells infected with cytomegalovirus to anti-tumor antibiotic doxorubicin and restoration the sensitivity by inhibitors of the molecular way PI3K/AKT/mTOR
Author(s) -
Ya. Yu. Chernoryzh,
Н. Е. Федорова,
Kirill I. Yurlov,
Ruslan A. Simonov,
А. Б. Корнев,
Dmitry S. Karpov,
Natalia F. Zakirova,
Alexander Ivanov,
А А Кущ,
А Л Гинцбург
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524894433-437
Subject(s) - pi3k/akt/mtor pathway , human cytomegalovirus , doxorubicin , protein kinase b , leukemia , thp1 cell line , drug resistance , protein synthesis inhibitor , cancer research , antibiotics , virology , medicine , biology , immunology , chemotherapy , cell culture , microbiology and biotechnology , phosphorylation , signal transduction , virus , antibacterial agent , genetics
Results obtained showed that infection with HCMV prevented the death of THP‑1 cells treated with DOX in both active and latent forms of infection. In the presence of mTOR inhibitors (rapamycin and Torin2), the sensitivity of the infected cells to DOX was restored. Rapamycin inhibited the expression of HCMV protein IE1-p72 and increased sensitivity to DOX. Molecular targets for the creation of new drugs for the treatment of leukemia in patients infected with HCMV have been determined.