Open AccessThe induction of the synthesis of interferon, CD4 and CD8 t lymphocytes in blood and spleen of mice after oral vaccination with the “early” protein HPV16 E2
Author(s) -
R. K. Salyaev,
N. I. Rekoslavskaya,
А. С. Столбиков
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524883333-337
Subject(s) - elispot , cd8 , spleen , immune system , peripheral blood mononuclear cell , splenocyte , immunology , interferon , vaccination , western blot , biology , cytotoxic t cell , antibody , virology , microbiology and biotechnology , gene , biochemistry , in vitro
Very high contents of the interferon, CD4 and CD8 T lymphocytes were found in blood and spleen cells after the oral vaccination of mice with the “early” protein of high-risk oncogenic papillomavirus HPV16 E2 by using immunoassays analyses: ELISPOT for the detection of the content of interferon, Western-blot hybridization and enzyme immunoassays for analyses of СD4 and CD8 T lymphocytes. Both blood cells and splenocytes showed high content of alive mononuclear cells during ELISA and ELISPOT analyses that had very characteristic features for T lymphocytes. The ratio of CD4/CD8 was very close to 1 after the quanttitive determination by the enzyme immunoassays with appropriate antibodies. It was concluded that the “early” protein HPV16 E2 participated in the activation of the immune system during pathogenesis and neoplasia.