Open AccessAn approach to better estimation of the intial velocity of enzyme reactions
Author(s) -
N. M. Malygina,
T. A. Petrova,
A. Y. Lianguzov,
А. М. Иванов
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524882225-228
Subject(s) - computation , reagent , kinetic energy , enzyme , lag , chemistry , enzyme assay , mathematics , biological system , algorithm , computer science , physics , biochemistry , biology , classical mechanics , computer network
A new algorithm for computation of initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce systematic error of measurements to the minimum, to estimate reaction velocity in testing samples regardless of the enzyme activity levels and to minimize assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag-phase in the kinetic curve.