Open AccessEffects of pinacidil and calcium on succinate-energized rat heart mitochondria in the presence of rotenone
Author(s) -
С. М. Коротков,
I. V. Brailovskaya,
В. П. Нестеров,
С. И. Сороко
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524874460-464
Subject(s) - pinacidil , rotenone , mitochondrion , calcium , chemistry , biophysics , swelling , membrane potential , inner mitochondrial membrane , stimulation , pharmacology , microbiology and biotechnology , medicine , biochemistry , endocrinology , biology , glibenclamide , organic chemistry , pathology , diabetes mellitus
The effect of pinacidil was studied on calcium-loaded rat heart mitochondria (RHM) in the presence of succinate and rotenone. In experiments with pinacidil, the swelling of these mitochondria increased in media with NH4NO3 or K‑acetate, but the inner membrane potential DΨmito and state 3 or 2,4-dinitrophenol-uncoupled respiration of these organelles were decreased due to opening of the mitochondrial permeability transition pore in the inner membrane. These effects were inhibited by cyclosporin A and ADP. It was concluded that the protective effect of pinacidil in the cardiac muscle ischemia/reperfusion may be associated with stimulation mitochondrial swelling and a decrease in RHM calcium overload resulted in a decrease in DΨmito due to the soft uncoupling pinacidil effect.