Open AccessThe development of reporter system for the investigation of molecular mechanisms of ecdysone response
Author(s) -
M. Yu. Mazina,
А. Н. Краснов,
Pavel Georgiev,
Nadezhda E. Vorobyeva
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524854515-518
Subject(s) - ecdysone , ecdysone receptor , reporter gene , construct (python library) , biology , computational biology , gene , microbiology and biotechnology , transcriptional regulation , gene expression , genetics , transcription factor , nuclear receptor , computer science , programming language
To study the mechanisms of transcriptional regulation, a convenient experimental approach is to use the artificial chimeric constructs, carrying the regulatory elements of interest. In the present work, we describe the creation and characterization of a novel genetic construct, which makes possible to study the transcriptional regulation of the early-late gene of the ecdysone cascade. Using the data of genome-wide experiments, we have isolated the main regulatory region of the hr4 gene, which was successfully used to create a chimeric reporter construct expressing a fluorescent protein upon the treatment with the ecdysone hormone. This reporter system can be used to study the mechanisms of the ecdysone response, both in cell culture and in tissues, at various stages of the Drosophila development.