Open AccessEndothelium-dependent hyperpolarization-mediated relaxation pathway in bovine mesenteric lymph nodes
Author(s) -
G. I. Lobov,
Д. П. Дворецкий
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524845645-648
Subject(s) - hyperpolarization (physics) , lymph , tetraethylammonium chloride , chemistry , prostacyclin , endothelium , tetraethylammonium , biophysics , mesenteric arteries , anatomy , microbiology and biotechnology , endocrinology , medicine , artery , pathology , biochemistry , biology , potassium , stereochemistry , organic chemistry , nuclear magnetic resonance spectroscopy
In vitro, endothelium-dependent relaxation mechanisms of smooth muscle cells of the bovine mesenteric lymph node capsule have been studied. The addition of L-NAME and indomethacin to physiological saline inhibited the production of endothelium NO and prostacyclin. In this solution, tetraethylammonium chloride and TRAM-34 increased the tone of the precontracted lymph nodes. Thus, in bovine mesenteric lymph nodes there is an relaxation mechanism mediated by endothelial hyperpolarization, realized by activating Ca2+-dependent K+-channels of large- and intermediate conductance.