Open AccessPersonalized approach to assessing mRNA expression of histidine-rich glycoprotein and immunohistochemical markers in diseases of the breast
Author(s) -
A. I. Autenshlyus,
А. В. Голованова,
А. А. Студеникина,
I. I. Brusentsov,
A. V. Proskura,
I. P. Zhurakovskiy,
S. A. Arkhipov,
S. V. Sidorov,
V. A. Vavilin,
V. V. Lyakhovich
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524845624-628
Subject(s) - immunohistochemistry , pathology , biopsy , breast carcinoma , epithelial–mesenchymal transition , breast cancer , messenger rna , breast biopsy , medicine , cancer research , biology , cancer , gene , metastasis , mammography , biochemistry
Biopsy material of patients with malignant and benign breast diseases was examined. HRG mRNA expression was detected in 70% of cases in biopsy material obtained from patients with nonspecific invasive carcinoma and in 66.7% of cases in biopsy material of patients with benign breast diseases. Immunohistochemical analysis revealed expression of collagen II, the beta-1 integrin, and E-cadherin – markers of epithelial–mesenchymal transition. The use of RT-qPCR combined with immunohistochemical study made it possible to identify atypical cells, which can be regarded as precancerous changes, in individual patients.