Open AccessSerotonin differentially modulates the functional properties of damaged and intact motoneurons of the frog spinal cord
Author(s) -
Natalia Kalinina,
Aleksey V. Zaitsev,
N. P. Vesselkin
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524843372-376
Subject(s) - afterhyperpolarization , spinal cord , neuroscience , electrophysiology , serotonin , membrane potential , chemistry , biology , anatomy , biophysics , receptor , biochemistry
The role of serotonin in the recovery of motor functions in spinal cord injuries is intensively studied, but the mechanism of its action remains unclear. In this work, we used the preparation of an isolated segment of the spinal cord of an adult frog to compare the electrophysiological properties of damaged and intact lumbar moto- neurons and the modulating effect of serotonin (5-HT) on them. Due to specific morphology of the motoneurons (a very branched dendritic tree), we could reliably obtain damaged (on the surface of the slice) and intact moto- neurons (in the depth of the slice). Using intracellular recording, we found significant differences between these groups of neurons in the resting membrane potential, input resistance, properties of the action potential (amplitude, duration, fast and medium phases of the afterhyperpolarization), the frequency of spikes. We found that 5-HT reduced the amplitude of the afterhyperpolarization and increased the frequency of spikes in intact neurons, whereas in damaged motoneurons, 5-HT increased the amplitude of the afterhyperpolarization and did not affect the frequency of discharges. The results of the study show that the properties of the motoneurons and the effect of neuromodulators on them, in particular, 5-HT, can change after damage.